The immune response to viral infection is a dynamic process, which is regulated by an intricate network of many genes and their products.
Understanding the dynamics of this network will infer the mechanisms involved in regulating influenza infection and hence aid the development of antiviral treatments and preventive vaccines. There has been an abundance of literature regarding dynamic network construction, e.g., Hecker et al. (2009), Lu et al. (2011) and Wu et al. (2013).
My research involves the development of a new pipeline for dynamic network construction for high-dimensional time course gene expression data. This pipeline allows us to discern the fundamental underlying biological process and their dynamic features at genetic level.
The pipeline includes:
Novel statistical methods and modelling approaches have been developed for the implementation of this new pipeline, which include a new approach for the selection of the smoothing parameter, a new clustering approach and a new method for model selection for high-dimensional ODEs.
Carey, M., Wu, S., Gan, G. and Wu, H. (2016) 'Correlation-based iterative clustering methods for time course data: the identification of temporal gene response modules to influenza infection in humans'. Infectious Disease Modelling.
Song, J., Carey, M., Zhu, H., Miao, H., Ramırez, Juan and Wu, H. (2017) 'Identifying the dynamic gene regulatory network during latent HIV-1reactivation using high-dimensional ordinary differential equations'. International Journal of Computational Biology and Drug Design
Carey, M., Wu, S., Wu, H., 'A big data pipeline: Identifying dynamic gene regulatory networks from time-course Gene Expression Omnibus data with applications to influenza infection'. Statistical Methods in Medical Research (in press)